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KMID : 0376219930300020177
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Chonnam Medical Journal
1993 Volume.30 No. 2 p.177 ~ p.186
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Mutagenicity of Ascorbic acid and Ferrous Sulfate in Salmonella typhimurium TA102 Strain
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Abstract
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In orther to investigate whether ascorbic acid, which helps transit ions to generate free radicals, could act as mutagen, mutagenicity of ascorbic acid and ferrous sulfate and their mechanisms were evaluated using preincubation
Salmonella/microsome
assay with Salmonella typhimurium TA102 strain. S. typhimurium TA102 strain is widely accepted as particularly useful one for the detection of oxidative mutagens. Ascorbic acid appeared to be mutagenic at the concentration of 30mM or more,
Ferrous
sulfate was also weakly mutagenic at the concentration of 0.05mM or more. The mutagenicity was synergistically potentiated when ascorbic acid and ferrous sulfate acted in concert. When EDTA was added to the preincubation mixture, mutagenicity of
ascorbic acid and ascorbic acid plus ferrous sulfate was completely blocked. Catalase also partially blocked the mutagenicity of ascorbic acid and ascorbic acid plus ferrous sulfate. Above results indicate that ascorbic acid in itself is non- or
scarcely mutagenic, but induce mutation by acting in concert with itself ions(such as ferrous ion ) contaminating the reaction mixture.
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